沈 华1，唐艺玲1，杜春燕1，胥 鑫1，于 思4，代 敏2，3，于月兰3*，蒲忠慧1，2*
（1.成都医学院检验医学院，四川成都 610500；
2.四川省动物源性食品兽药残留防控技术工程实验室，四川成都 610500；
3.成都医学院人事处，四川成都 610500；
4.成都医学院教务处，四川成都 610500）
摘要：分析比较不同提取方法制备的草果挥发油成分。分别采用水蒸气蒸馏法和超临界CO2萃取法制备草果挥发油，通过气质联用技术（GC-MS）分析不同提取物的化学成分。水蒸气蒸馏法和超临界CO2萃取法提取草果挥发油得率分别为1.65%和2.18%，两种方法制备的草果挥发油成分类型和相对含量差异显著，水蒸气蒸馏法制备的挥发油共检测出37个化学成分，其中单萜类成分约占85.20%，倍半萜类成分约占4.77%；超临界CO2萃取法制备的挥发油共鉴定43个化学成分，单萜类成分约占82.54%，倍半萜类成分约占6.36%。两种方法提取的草果油化学成分和相对含量均有一定差异，水蒸气蒸馏法对草果挥发油中的单萜类保留相对较强，超临界CO2萃取法对倍半萜类成分的提取率相对较高。
关键词：草果；挥发油；GC-MS
中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2021）03-0145-0007
Gas Chromatography-Mass Spectrometry Analysis for Volatile Oil from Amomum tsao-ko by Different Extraction Methods
SHEN Hua1, TANG Yi-ling1, DU Chun-yan1, XU Xin1, YU Si4，DAI Min2，3, YU Yue-lan3*, PU Zhong-hui1，2*
（1.School of Laboratory Medicine, Chengdu Medical College, Chengdu Sichuan 610500, China;
2.Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food, Chengdu Sichuan 610500, China;
3.Personnel Office of Chengdu Medical College, Chengdu Sichuan 610500, China;
4.Teaching Affairs Department, Chengdu Medical College, Chengdu Sichuan 610500, China）
Abstract：To compare and analyze essential oil of Amomum tsao- ko extracted by different methods. The essential oil of Amomum tsao-ko were extracted by means of supercritical CO2 extraction and steam distillation respectively, and the obtained samples were analyzed by gas chromatography-mass spectrometry (GC-MS). The yields of volatile oils extracted by steam distillation and CO2 supercritical extraction were 1.65% and 2.18%, respectively. The type and relative content of volatile oil were significantly different. The essential oil extracted by steam distillation method contained 37 chemical components, among which the monoterpenoids account for 85.20% of the total essential oil, and the sesquiterpenes components account for 4.77% of the total essential oil. And 43 compounds were identified from essential oil extracted by CO2 supercriticalextraction，with monoterpenoids accounting for 82.54% of total essential oil and sesquiterpenes 6.36%. The types of chemical composition and relative contents of the essential oil of Amomum tsao-ko were varied by the two methods. The monoterpenoids of the essential oil were retained relatively strong by steam distillation method, while the sesquiterpeneshad a relatively high extraction rate by the CO2 supercritical fluid extraction.
Keywords：Amomum tsao-ko; volatile oil; GC-MS
doi：10.3969/j.issn.1674-506X.2021.03-023
收稿日期：2020-12-01
基金项目：发育与再生四川省重点实验室研究基金（No.SYS19-08）；成都医学院自然科学一般项目（CYZ18-18）；四川省省级大学生创新创业训练计划项目（No.201913705018）。
作者简介：沈华（1998-），男，本科。研究方向：天然药物提取、分离与纯化。
*通信作者
引用格式：沈华,唐艺玲,杜春燕,等.不同方法提取草果油的GC-MS分析[J].食品与发酵科技,2021,57(3):145-151.
